Stats by Source
Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)
Total = all mutations for this gene across every source.
Cell line = COSMIC Cell Lines Project + DepMap + PubMed.
Tissue = COSMIC primary-tissue (patient tumour) samples.
Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.
| Total | Cell line | Tissue | |
|---|---|---|---|
| Mutations | 444 | 77 | 366 |
| Samples | 156 | 37 | 118 |
| Peptides | 127 | 26 | 104 |
Function
ATF4 · Activating transcription factor 4
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011].
Isoforms & Proteins
4 transcripts · UniProt mapping is sequence-verified (AA-safe)
Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.
The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.
Counts are mutations and unique mutant peptides on each transcript.
Gene Properties
Recurrent Mutations
All 121 amino-acid changes on canonical ENST00000396680 · needle height = samples · drag the mini-map to zoom
A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).
X-axis = amino-acid position in the protein.
Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.
The most recurrent changes are labelled; hover any needle for the change, position and counts.
Mutation frequency across cancer types
% of samples with a missense/complex mutation in ATF4 · cell line vs tissue
For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in ATF4 – counted as distinct samples (a sample counts once no matter how many mutations it has).
Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.
| Cancer type | Cell lines | Tissue samples |
|---|---|---|
| T-Lymphoblastic Leukemia | 5/40 12% | 0/0 0% |
| Glioblastoma | 2/98 2% | 0/0 0% |
| Endometrial Carcinoma | 2/42 5% | 6/612 1% |
| Melanoma | 0/210 0% | 24/1899 1% |
| Bladder Carcinoma | 0/58 0% | 11/956 1% |
| Burkitts Lymphoma | 0/32 0% | 2/196 1% |
| Squamous Cell Lung Carcinoma | 1/57 2% | 6/810 1% |
| Gastrointestinal Stromal Tumour | 0/0 0% | 1/133 1% |
| Cervical Carcinoma | 0/35 0% | 3/422 1% |
| Neuroendocrine Tumour | 2/154 1% | 1/577 0% |
| Non-Small Cell Lung Carcinoma | 2/304 1% | 5/1390 0% |
| Esophageal Carcinoma | 0/23 0% | 3/769 0% |
| Other Solid Cancers | 2/94 2% | 4/1515 0% |
| Head and Neck Carcinoma | 1/85 1% | 5/1574 0% |
| Colorectal Carcinoma | 3/143 2% | 9/3239 0% |
| Breast Carcinoma | 4/144 3% | 8/3264 0% |
| Non-Cancerous | 3/104 3% | 0/830 0% |
| Ewings Sarcoma | 1/63 2% | 0/262 0% |
| Other Blood Cancers | 1/61 2% | 7/2725 0% |
| Pancreatic Carcinoma | 2/89 2% | 2/1611 0% |
| B-Cell Non-Hodgkins Lymphoma | 2/88 2% | 4/2534 0% |
| Glioma | 0/52 0% | 4/2127 0% |
| Hepatocellular Carcinoma | 0/46 0% | 4/2210 0% |
| Gastric Carcinoma | 1/74 1% | 2/1809 0% |
| Kidney Carcinoma | 0/85 0% | 3/1862 0% |
| Neuroblastoma | 2/87 2% | 0/1331 0% |
| Esophageal Squamous Cell Carcinoma | 0/51 0% | 3/2550 0% |
| Prostate Carcinoma | 1/13 8% | 1/2105 0% |
| Thyroid Gland Carcinoma | 0/45 0% | 1/1592 0% |
Mutation Distribution
Where ATF4 is mutated · all tissues, split by cell line vs tissue
How many mutations in ATF4 were found in each tissue, across the whole database.
Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.
This shows the cancer-context where this gene is recurrently altered.
GTEx Expression
Median TPM across 54 healthy tissues
Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.
Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.
Scroll or drag the mini-axis below the chart to browse all tissues.
Mutations
All 444 mutations in ATF4
Every mutation record for this gene, across all samples and sources.
The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).
Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.
| ID | Sample | Transcript | AA Change | CDS | Type | Source | Mutant Peptide | Wild-type Peptide |
|---|