Stats by Source
Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)
Total = all mutations for this gene across every source.
Cell line = COSMIC Cell Lines Project + DepMap + PubMed.
Tissue = COSMIC primary-tissue (patient tumour) samples.
Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.
| Total | Cell line | Tissue | |
|---|---|---|---|
| Mutations | 1,573 | 171 | 1,359 |
| Samples | 458 | 82 | 361 |
| Peptides | 384 | 58 | 335 |
Function
BEST3 · Bestrophin 3
BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]).[supplied by OMIM, Mar 2008].
Isoforms & Proteins
8 transcripts · UniProt mapping is sequence-verified (AA-safe)
Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.
The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.
Counts are mutations and unique mutant peptides on each transcript.
Gene Properties
Recurrent Mutations
All 341 amino-acid changes on canonical ENST00000330891 · needle height = samples · drag the mini-map to zoom
A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).
X-axis = amino-acid position in the protein.
Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.
The most recurrent changes are labelled; hover any needle for the change, position and counts.
Mutation frequency across cancer types
% of samples with a missense/complex mutation in BEST3 · cell line vs tissue
For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in BEST3 – counted as distinct samples (a sample counts once no matter how many mutations it has).
Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.
| Cancer type | Cell lines | Tissue samples |
|---|---|---|
| Melanoma | 8/210 4% | 96/1899 5% |
| Oral Cavity Carcinoma | 2/54 4% | 0/0 0% |
| Endometrial Carcinoma | 4/42 10% | 17/612 3% |
| T-Lymphoblastic Leukemia | 1/40 2% | 0/0 0% |
| Cervical Carcinoma | 2/35 6% | 6/422 1% |
| Non-Small Cell Lung Carcinoma | 3/304 1% | 25/1390 2% |
| Colorectal Carcinoma | 11/143 8% | 44/3239 1% |
| Bladder Carcinoma | 2/58 3% | 14/956 1% |
| Germ Cell Tumour | 1/25 4% | 2/169 1% |
| Gastrointestinal Stromal Tumour | 0/0 0% | 2/133 2% |
| Biliary Tract Carcinoma | 0/54 0% | 14/950 1% |
| Other Solid Cancers | 1/94 1% | 21/1515 1% |
| Gastric Carcinoma | 1/74 1% | 21/1809 1% |
| Squamous Cell Lung Carcinoma | 0/57 0% | 10/810 1% |
| Burkitts Lymphoma | 2/32 6% | 0/196 0% |
| Meningioma | 0/3 0% | 2/252 1% |
| Breast Carcinoma | 7/144 5% | 19/3264 1% |
| Hodgkins Lymphoma | 1/16 6% | 0/122 0% |
| Head and Neck Carcinoma | 4/85 5% | 8/1574 1% |
| Hepatocellular Carcinoma | 1/46 2% | 14/2210 1% |
| Small Cell Lung Carcinoma | 2/9 22% | 3/752 0% |
| Other Sarcomas | 0/69 0% | 5/699 1% |
| Non-Cancerous | 1/104 1% | 4/830 0% |
| Rhabdomyosarcoma | 0/33 0% | 1/171 1% |
| Neuroblastoma | 4/87 5% | 3/1331 0% |
| Osteosarcoma | 0/45 0% | 1/166 1% |
| Wilms Tumour | 1/5 20% | 1/474 0% |
| Pancreatic Carcinoma | 2/89 2% | 5/1611 0% |
| Neuroendocrine Tumour | 1/154 1% | 2/577 0% |
| Prostate Carcinoma | 4/13 31% | 4/2105 0% |
Mutation Distribution
Where BEST3 is mutated · all tissues, split by cell line vs tissue
How many mutations in BEST3 were found in each tissue, across the whole database.
Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.
This shows the cancer-context where this gene is recurrently altered.
GTEx Expression
Median TPM across 48 healthy tissues
Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.
Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.
Scroll or drag the mini-axis below the chart to browse all tissues.
Mutations
All 1,573 mutations in BEST3
Every mutation record for this gene, across all samples and sources.
The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).
Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.
| ID | Sample | Transcript | AA Change | CDS | Type | Source | Mutant Peptide | Wild-type Peptide |
|---|