CASS4

Cas scaffold protein family member 4 Q9NQ75 CASS4_HUMAN
Protein Coding Chr 20 20q13.31 Swiss-Prot reviewed Entrez 57091
Mutations
964
CL 148 · Tissue 801
Samples
641
CL 120 · Tissue 512
Peptides
499
unique mutant peptides
Transcripts
3
isoforms mutated

Stats by Source

Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)

How the counts split by source
Stats by Source

Total = all mutations for this gene across every source.

Cell line = COSMIC Cell Lines Project + DepMap + PubMed.

Tissue = COSMIC primary-tissue (patient tumour) samples.

Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.

TotalCell lineTissue
Mutations964148801
Samples641120512
Peptides49976435

Function

CASS4 · Cas scaffold protein family member 4

Enables protein tyrosine kinase binding activity. Involved in several processes, including positive regulation of protein kinase B signaling; positive regulation of protein tyrosine kinase activity; and positive regulation of substrate adhesion-dependent cell spreading. Located in focal adhesion. Part of cytoplasm. [provided by Alliance of Genome Resources, Apr 2022]

Isoforms & Proteins

3 transcripts · UniProt mapping is sequence-verified (AA-safe)

About the isoform mapping
Isoforms & Proteins

Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.

The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.

Counts are mutations and unique mutant peptides on each transcript.

TranscriptUniProtMutationsPeptides
ENST00000360314 Q9NQ75 629 463
ENST00000434344 Q9NQ75-3 273 197
ENST00000679887 Q9NQ75 62 54

Gene Properties

Type
Protein Coding
Chromosome
20
Cytoband
20q13.31
Entrez ID
Aliases
C20orf32CAS4HEFLHEPL

Recurrent Mutations

All 463 amino-acid changes on canonical ENST00000360314 · needle height = samples · drag the mini-map to zoom

What this lollipop shows
Recurrent Mutations

A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).

X-axis = amino-acid position in the protein.

Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.

The most recurrent changes are labelled; hover any needle for the change, position and counts.

Mutation frequency across cancer types

% of samples with a missense/complex mutation in CASS4 · cell line vs tissue

How this frequency is counted
Cancer-type mutation frequency

For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in CASS4 – counted as distinct samples (a sample counts once no matter how many mutations it has).

Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.

Cancer typeCell linesTissue samples
Endometrial Carcinoma
6/42 14%
31/612 5%
Melanoma
16/210 8%
101/1899 5%
T-Lymphoblastic Leukemia
2/40 5%
0/0 0%
Chronic Myelogenous Leukemia
1/25 4%
0/0 0%
T-Cell Non-Hodgkins Lymphoma
1/26 4%
0/0 0%
Non-Small Cell Lung Carcinoma
20/304 7%
40/1390 3%
Acute Myeloid Leukemia
3/90 3%
0/0 0%
Glioblastoma
3/98 3%
0/0 0%
Squamous Cell Lung Carcinoma
9/57 16%
16/810 2%
Colorectal Carcinoma
25/143 17%
67/3239 2%
Gastric Carcinoma
0/74 0%
47/1809 3%
Gastrointestinal Stromal Tumour
0/0 0%
3/133 2%
Rhabdomyosarcoma
2/33 6%
2/171 1%
Other Solid Cancers
0/94 0%
27/1515 2%
Small Cell Lung Carcinoma
0/9 0%
11/752 1%
Neuroendocrine Tumour
6/154 4%
3/577 1%
Head and Neck Carcinoma
2/85 2%
16/1574 1%
Esophageal Squamous Cell Carcinoma
0/51 0%
27/2550 1%
Ovarian Carcinoma
3/109 3%
8/998 1%
Burkitts Lymphoma
2/32 6%
0/196 0%
Cervical Carcinoma
0/35 0%
4/422 1%
Pancreatic Carcinoma
3/89 3%
11/1611 1%
Other Sarcomas
2/69 3%
4/699 1%
Glioma
0/52 0%
16/2127 1%
Biliary Tract Carcinoma
1/54 2%
6/950 1%
Breast Carcinoma
3/144 2%
19/3264 1%
Ewings Sarcoma
0/63 0%
2/262 1%
Hepatocellular Carcinoma
0/46 0%
13/2210 1%
Plasma Cell Myeloma
0/44 0%
2/305 1%
Esophageal Carcinoma
0/23 0%
4/769 1%

Mutation Distribution

Where CASS4 is mutated · all tissues, split by cell line vs tissue

Mutation counts by tissue
Mutation Distribution

How many mutations in CASS4 were found in each tissue, across the whole database.

Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.

This shows the cancer-context where this gene is recurrently altered.

GTEx Expression

Median TPM across 54 healthy tissues

GTEx Portal ↗
About the expression data
GTEx Expression

Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.

Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.

Scroll or drag the mini-axis below the chart to browse all tissues.

Mutations

All 964 mutations in CASS4

About the mutation list
Mutations

Every mutation record for this gene, across all samples and sources.

The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).

Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.

IDSampleTranscriptAA Change CDSTypeSourceMutant PeptideWild-type Peptide