Stats by Source
Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)
Total = all mutations for this gene across every source.
Cell line = COSMIC Cell Lines Project + DepMap + PubMed.
Tissue = COSMIC primary-tissue (patient tumour) samples.
Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.
| Total | Cell line | Tissue | |
|---|---|---|---|
| Mutations | 736 | 135 | 590 |
| Samples | 346 | 83 | 256 |
| Peptides | 328 | 60 | 274 |
Function
MCM3 · Minichromosome maintenance complex component 3
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018].
Isoforms & Proteins
4 transcripts · UniProt mapping is sequence-verified (AA-safe)
Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.
The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.
Counts are mutations and unique mutant peptides on each transcript.
| Transcript | UniProt | Mutations | Peptides |
|---|---|---|---|
| ENST00000596288 | P25205 | 359 | 264 |
| ENST00000616552 | P25205-2 | 336 | 254 |
| ENST00000229854 | A0A499FHX9* | 22 | 17 |
| ENST00000419835 | J3KQ69* | 19 | 14 |
Gene Properties
Recurrent Mutations
All 264 amino-acid changes on canonical ENST00000596288 · needle height = samples · drag the mini-map to zoom
A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).
X-axis = amino-acid position in the protein.
Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.
The most recurrent changes are labelled; hover any needle for the change, position and counts.
Mutation frequency across cancer types
% of samples with a missense/complex mutation in MCM3 · cell line vs tissue
For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in MCM3 – counted as distinct samples (a sample counts once no matter how many mutations it has).
Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.
| Cancer type | Cell lines | Tissue samples |
|---|---|---|
| T-Lymphoblastic Leukemia | 4/40 10% | 0/0 0% |
| Chronic Myelogenous Leukemia | 2/25 8% | 0/0 0% |
| Endometrial Carcinoma | 5/42 12% | 20/612 3% |
| Glioblastoma | 3/98 3% | 0/0 0% |
| Melanoma | 5/210 2% | 35/1899 2% |
| Colorectal Carcinoma | 12/143 8% | 40/3239 1% |
| Non-Small Cell Lung Carcinoma | 4/304 1% | 20/1390 1% |
| Burkitts Lymphoma | 2/32 6% | 1/196 1% |
| Acute Myeloid Leukemia | 1/90 1% | 0/0 0% |
| Gastric Carcinoma | 4/74 5% | 14/1809 1% |
| Squamous Cell Lung Carcinoma | 3/57 5% | 5/810 1% |
| Thyroid Gland Carcinoma | 0/45 0% | 15/1592 1% |
| Plasma Cell Myeloma | 2/44 5% | 1/305 0% |
| Neuroendocrine Tumour | 2/154 1% | 4/577 1% |
| Esophageal Carcinoma | 1/23 4% | 5/769 1% |
| Non-Cancerous | 2/104 2% | 5/830 1% |
| Ovarian Carcinoma | 4/109 4% | 4/998 0% |
| Hodgkins Lymphoma | 0/16 0% | 1/122 1% |
| Other Solid Cancers | 1/94 1% | 10/1515 1% |
| Cervical Carcinoma | 2/35 6% | 1/422 0% |
| Pancreatic Carcinoma | 6/89 7% | 5/1611 0% |
| Other Sarcomas | 1/69 1% | 4/699 1% |
| Ewings Sarcoma | 0/63 0% | 2/262 1% |
| Bladder Carcinoma | 0/58 0% | 6/956 1% |
| Small Cell Lung Carcinoma | 0/9 0% | 4/752 1% |
| Hepatocellular Carcinoma | 0/46 0% | 12/2210 1% |
| Glioma | 2/52 4% | 9/2127 0% |
| Rhabdomyosarcoma | 1/33 3% | 0/171 0% |
| Head and Neck Carcinoma | 1/85 1% | 7/1574 0% |
| Osteosarcoma | 0/45 0% | 1/166 1% |
Mutation Distribution
Where MCM3 is mutated · all tissues, split by cell line vs tissue
How many mutations in MCM3 were found in each tissue, across the whole database.
Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.
This shows the cancer-context where this gene is recurrently altered.
GTEx Expression
Median TPM across 54 healthy tissues
Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.
Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.
Scroll or drag the mini-axis below the chart to browse all tissues.
Mutations
All 736 mutations in MCM3
Every mutation record for this gene, across all samples and sources.
The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).
Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.
| ID | Sample | Transcript | AA Change | CDS | Type | Source | Mutant Peptide | Wild-type Peptide |
|---|