Stats by Source
Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)
Total = all mutations for this gene across every source.
Cell line = COSMIC Cell Lines Project + DepMap + PubMed.
Tissue = COSMIC primary-tissue (patient tumour) samples.
Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.
| Total | Cell line | Tissue | |
|---|---|---|---|
| Mutations | 1,424 | 234 | 1,162 |
| Samples | 505 | 108 | 392 |
| Peptides | 428 | 75 | 355 |
Function
MLH3 · MutL homolog 3
This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008].
Isoforms & Proteins
3 transcripts · UniProt mapping is sequence-verified (AA-safe)
Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.
The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.
Counts are mutations and unique mutant peptides on each transcript.
Gene Properties
Recurrent Mutations
All 413 amino-acid changes on canonical ENST00000355774 · needle height = samples · drag the mini-map to zoom
A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).
X-axis = amino-acid position in the protein.
Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.
The most recurrent changes are labelled; hover any needle for the change, position and counts.
Mutation frequency across cancer types
% of samples with a missense/complex mutation in MLH3 · cell line vs tissue
For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in MLH3 – counted as distinct samples (a sample counts once no matter how many mutations it has).
Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.
| Cancer type | Cell lines | Tissue samples |
|---|---|---|
| T-Lymphoblastic Leukemia | 6/40 15% | 0/0 0% |
| Chronic Myelogenous Leukemia | 2/25 8% | 0/0 0% |
| Endometrial Carcinoma | 6/42 14% | 28/612 5% |
| Gastrointestinal Stromal Tumour | 0/0 0% | 4/133 3% |
| Other Solid Cancers | 2/94 2% | 43/1515 3% |
| Acute Myeloid Leukemia | 2/90 2% | 0/0 0% |
| Melanoma | 9/210 4% | 37/1899 2% |
| Germ Cell Tumour | 2/25 8% | 2/169 1% |
| Glioblastoma | 2/98 2% | 0/0 0% |
| Colorectal Carcinoma | 11/143 8% | 50/3239 2% |
| Neuroendocrine Tumour | 10/154 6% | 3/577 1% |
| Cervical Carcinoma | 0/35 0% | 8/422 2% |
| Squamous Cell Lung Carcinoma | 3/57 5% | 12/810 1% |
| Bladder Carcinoma | 2/58 3% | 15/956 2% |
| Non-Small Cell Lung Carcinoma | 11/304 4% | 16/1390 1% |
| Hodgkins Lymphoma | 0/16 0% | 2/122 2% |
| Gastric Carcinoma | 5/74 7% | 22/1809 1% |
| Plasma Cell Myeloma | 3/44 7% | 1/305 0% |
| Head and Neck Carcinoma | 3/85 4% | 15/1574 1% |
| Hepatocellular Carcinoma | 4/46 9% | 20/2210 1% |
| Ovarian Carcinoma | 0/109 0% | 9/998 1% |
| Breast Carcinoma | 4/144 3% | 21/3264 1% |
| Glioma | 0/52 0% | 15/2127 1% |
| Prostate Carcinoma | 2/13 15% | 12/2105 1% |
| Small Cell Lung Carcinoma | 0/9 0% | 5/752 1% |
| Pancreatic Carcinoma | 1/89 1% | 9/1611 1% |
| Esophageal Squamous Cell Carcinoma | 2/51 4% | 13/2550 1% |
| Rhabdomyosarcoma | 0/33 0% | 1/171 1% |
| B-Cell Non-Hodgkins Lymphoma | 3/88 3% | 9/2534 0% |
| Burkitts Lymphoma | 1/32 3% | 0/196 0% |
Mutation Distribution
Where MLH3 is mutated · all tissues, split by cell line vs tissue
How many mutations in MLH3 were found in each tissue, across the whole database.
Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.
This shows the cancer-context where this gene is recurrently altered.
GTEx Expression
Median TPM across 54 healthy tissues
Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.
Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.
Scroll or drag the mini-axis below the chart to browse all tissues.
Mutations
All 1,424 mutations in MLH3
Every mutation record for this gene, across all samples and sources.
The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).
Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.
| ID | Sample | Transcript | AA Change | CDS | Type | Source | Mutant Peptide | Wild-type Peptide |
|---|