NDUFAF3

NADH:ubiquinone oxidoreductase complex assembly factor 3 Q9BU61 NDUF3_HUMAN
Protein Coding Chr 3 3p21.31 Swiss-Prot reviewed Entrez 25915
Mutations
171
CL 29 · Tissue 142
Samples
64
CL 17 · Tissue 47
Peptides
60
unique mutant peptides
Transcripts
4
isoforms mutated

Stats by Source

Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)

How the counts split by source
Stats by Source

Total = all mutations for this gene across every source.

Cell line = COSMIC Cell Lines Project + DepMap + PubMed.

Tissue = COSMIC primary-tissue (patient tumour) samples.

Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.

TotalCell lineTissue
Mutations17129142
Samples641747
Peptides601249

Function

NDUFAF3 · NADH:ubiquinone oxidoreductase complex assembly factor 3

This gene encodes a mitochondrial complex I assembly protein that interacts with complex I subunits. Mutations in this gene cause mitochondrial complex I deficiency, a fatal neonatal disorder of the oxidative phosphorylation system. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2009].

Isoforms & Proteins

4 transcripts · UniProt mapping is sequence-verified (AA-safe)

About the isoform mapping
Isoforms & Proteins

Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.

The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.

Counts are mutations and unique mutant peptides on each transcript.

TranscriptUniProtMutationsPeptides
ENST00000326925 Q9BU61 69 54
ENST00000326912 Q9BU61-2 34 30
ENST00000395458 Q9BU61-2 34 30
ENST00000451378 Q9BU61-2 34 30

Gene Properties

Type
Protein Coding
Chromosome
3
Cytoband
3p21.31
Entrez ID
Aliases
2P1C3orf60E3-3MC1DN18

Recurrent Mutations

All 54 amino-acid changes on canonical ENST00000326925 · needle height = samples · drag the mini-map to zoom

What this lollipop shows
Recurrent Mutations

A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).

X-axis = amino-acid position in the protein.

Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.

The most recurrent changes are labelled; hover any needle for the change, position and counts.

Mutation frequency across cancer types

% of samples with a missense/complex mutation in NDUFAF3 · cell line vs tissue

How this frequency is counted
Cancer-type mutation frequency

For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in NDUFAF3 – counted as distinct samples (a sample counts once no matter how many mutations it has).

Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.

Cancer typeCell linesTissue samples
Burkitts Lymphoma
2/32 6%
0/196 0%
Bladder Carcinoma
1/58 2%
7/956 1%
Mesothelioma
1/62 2%
0/165 0%
Cervical Carcinoma
0/35 0%
2/422 0%
Thyroid Gland Carcinoma
0/45 0%
6/1592 0%
Colorectal Carcinoma
5/143 4%
7/3239 0%
Melanoma
3/210 1%
4/1899 0%
Plasma Cell Myeloma
0/44 0%
1/305 0%
Wilms Tumour
0/5 0%
1/474 0%
Endometrial Carcinoma
0/42 0%
1/612 0%
Kidney Carcinoma
0/85 0%
3/1862 0%
Neuroblastoma
2/87 2%
0/1331 0%
Head and Neck Carcinoma
0/85 0%
2/1574 0%
Non-Small Cell Lung Carcinoma
0/304 0%
2/1390 0%
Squamous Cell Lung Carcinoma
0/57 0%
1/810 0%
B-Cell Non-Hodgkins Lymphoma
0/88 0%
3/2534 0%
Gastric Carcinoma
1/74 1%
1/1809 0%
Glioma
1/52 2%
1/2127 0%
Breast Carcinoma
0/144 0%
2/3264 0%
Pancreatic Carcinoma
0/89 0%
1/1611 0%
Other Solid Cancers
0/94 0%
1/1515 0%
Esophageal Squamous Cell Carcinoma
0/51 0%
1/2550 0%
B-Lymphoblastic Leukemia
1/55 2%
0/2640 0%

Mutation Distribution

Where NDUFAF3 is mutated · all tissues, split by cell line vs tissue

Mutation counts by tissue
Mutation Distribution

How many mutations in NDUFAF3 were found in each tissue, across the whole database.

Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.

This shows the cancer-context where this gene is recurrently altered.

GTEx Expression

Median TPM across 54 healthy tissues

GTEx Portal ↗
About the expression data
GTEx Expression

Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.

Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.

Scroll or drag the mini-axis below the chart to browse all tissues.

Mutations

All 171 mutations in NDUFAF3

About the mutation list
Mutations

Every mutation record for this gene, across all samples and sources.

The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).

Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.

IDSampleTranscriptAA Change CDSTypeSourceMutant PeptideWild-type Peptide