Stats by Source
Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)
Total = all mutations for this gene across every source.
Cell line = COSMIC Cell Lines Project + DepMap + PubMed.
Tissue = COSMIC primary-tissue (patient tumour) samples.
Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.
| Total | Cell line | Tissue | |
|---|---|---|---|
| Mutations | 500 | 104 | 387 |
| Samples | 176 | 51 | 121 |
| Peptides | 147 | 40 | 107 |
Function
NEK3 · NIMA related kinase 3
This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Two functional alleles for this gene have been identified in humans. The reference genome assembly (GRCh38) represents a functional allele that is associated with the inclusion of an additional coding exon in protein-coding transcripts, compared to an alternate functional allele that lacks the exon. [provided by RefSeq, Sep 2019].
Isoforms & Proteins
4 transcripts · UniProt mapping is sequence-verified (AA-safe)
Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.
The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.
Counts are mutations and unique mutant peptides on each transcript.
| Transcript | UniProt | Mutations | Peptides |
|---|---|---|---|
| ENST00000610828 | P51956 | 188 | 143 |
| ENST00000618534 | P51956 | 150 | 124 |
| ENST00000620675 | A0A087WY58* | 149 | 123 |
| ENST00000629912 | F8VTT4* | 13 | 9 |
Gene Properties
Recurrent Mutations
All 143 amino-acid changes on canonical ENST00000610828 · needle height = samples · drag the mini-map to zoom
A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).
X-axis = amino-acid position in the protein.
Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.
The most recurrent changes are labelled; hover any needle for the change, position and counts.
Mutation frequency across cancer types
% of samples with a missense/complex mutation in NEK3 · cell line vs tissue
For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in NEK3 – counted as distinct samples (a sample counts once no matter how many mutations it has).
Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.
| Cancer type | Cell lines | Tissue samples |
|---|---|---|
| Oral Cavity Carcinoma | 2/54 4% | 0/0 0% |
| Chondrosarcoma | 2/14 14% | 0/75 0% |
| Endometrial Carcinoma | 3/42 7% | 10/612 2% |
| Rhabdomyosarcoma | 2/33 6% | 1/171 1% |
| Acute Myeloid Leukemia | 1/90 1% | 0/0 0% |
| Glioblastoma | 1/98 1% | 0/0 0% |
| Colorectal Carcinoma | 7/143 5% | 22/3239 1% |
| Melanoma | 2/210 1% | 16/1899 1% |
| Other Solid Cancers | 1/94 1% | 11/1515 1% |
| Hodgkins Lymphoma | 1/16 6% | 0/122 0% |
| Ovarian Carcinoma | 4/109 4% | 2/998 0% |
| Gastric Carcinoma | 3/74 4% | 7/1809 0% |
| Thyroid Gland Carcinoma | 0/45 0% | 8/1592 0% |
| Bladder Carcinoma | 2/58 3% | 3/956 0% |
| Non-Small Cell Lung Carcinoma | 2/304 1% | 6/1390 0% |
| Burkitts Lymphoma | 1/32 3% | 0/196 0% |
| Head and Neck Carcinoma | 2/85 2% | 4/1574 0% |
| Glioma | 0/52 0% | 7/2127 0% |
| Non-Cancerous | 1/104 1% | 2/830 0% |
| Plasma Cell Myeloma | 1/44 2% | 0/305 0% |
| Kidney Carcinoma | 1/85 1% | 4/1862 0% |
| Esophageal Carcinoma | 0/23 0% | 2/769 0% |
| Esophageal Squamous Cell Carcinoma | 3/51 6% | 3/2550 0% |
| Wilms Tumour | 0/5 0% | 1/474 0% |
| Neuroblastoma | 2/87 2% | 1/1331 0% |
| Other Blood Cancers | 4/61 7% | 1/2725 0% |
| Breast Carcinoma | 0/144 0% | 5/3264 0% |
| Neuroendocrine Tumour | 1/154 1% | 0/577 0% |
| Other Sarcomas | 0/69 0% | 1/699 0% |
| Hepatocellular Carcinoma | 0/46 0% | 3/2210 0% |
Mutation Distribution
Where NEK3 is mutated · all tissues, split by cell line vs tissue
How many mutations in NEK3 were found in each tissue, across the whole database.
Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.
This shows the cancer-context where this gene is recurrently altered.
GTEx Expression
Median TPM across 54 healthy tissues
Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.
Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.
Scroll or drag the mini-axis below the chart to browse all tissues.
Mutations
All 500 mutations in NEK3
Every mutation record for this gene, across all samples and sources.
The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).
Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.
| ID | Sample | Transcript | AA Change | CDS | Type | Source | Mutant Peptide | Wild-type Peptide |
|---|