PGK1

Phosphoglycerate kinase 1 P00558 PGK1_HUMAN
Protein Coding Chr X Xq21.1 Swiss-Prot reviewed Entrez 5230
Mutations
314
CL 37 · Tissue 270
Samples
159
CL 24 · Tissue 130
Peptides
141
unique mutant peptides
Transcripts
2
isoforms mutated

Stats by Source

Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)

How the counts split by source
Stats by Source

Total = all mutations for this gene across every source.

Cell line = COSMIC Cell Lines Project + DepMap + PubMed.

Tissue = COSMIC primary-tissue (patient tumour) samples.

Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.

TotalCell lineTissue
Mutations31437270
Samples15924130
Peptides14117123

Function

PGK1 · Phosphoglycerate kinase 1

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014].

Isoforms & Proteins

2 transcripts · UniProt mapping is sequence-verified (AA-safe)

About the isoform mapping
Isoforms & Proteins

Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.

The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.

Counts are mutations and unique mutant peptides on each transcript.

TranscriptUniProtMutationsPeptides
ENST00000373316 P00558 170 136
ENST00000644362 P00558-2 144 121

Gene Properties

Type
Protein Coding
Chromosome
X
Cytoband
Xq21.1
Entrez ID
Aliases
HEL-S-68pMIG10PGKA

Recurrent Mutations

All 136 amino-acid changes on canonical ENST00000373316 · needle height = samples · drag the mini-map to zoom

What this lollipop shows
Recurrent Mutations

A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).

X-axis = amino-acid position in the protein.

Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.

The most recurrent changes are labelled; hover any needle for the change, position and counts.

Mutation frequency across cancer types

% of samples with a missense/complex mutation in PGK1 · cell line vs tissue

How this frequency is counted
Cancer-type mutation frequency

For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in PGK1 – counted as distinct samples (a sample counts once no matter how many mutations it has).

Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.

Cancer typeCell linesTissue samples
Endometrial Carcinoma
1/42 2%
15/612 2%
Glioblastoma
1/98 1%
0/0 0%
Burkitts Lymphoma
2/32 6%
0/196 0%
Gastric Carcinoma
1/74 1%
14/1809 1%
Non-Small Cell Lung Carcinoma
4/304 1%
8/1390 1%
Melanoma
2/210 1%
13/1899 1%
Colorectal Carcinoma
4/143 3%
17/3239 1%
Thyroid Gland Carcinoma
0/45 0%
10/1592 1%
Bladder Carcinoma
0/58 0%
6/956 1%
Other Sarcomas
2/69 3%
2/699 0%
Other Solid Cancers
2/94 2%
6/1515 0%
Ovarian Carcinoma
1/109 1%
4/998 0%
Medulloblastoma
0/0 0%
2/450 0%
Cervical Carcinoma
0/35 0%
2/422 0%
Glioma
0/52 0%
8/2127 0%
Breast Carcinoma
2/144 1%
8/3264 0%
Neuroendocrine Tumour
2/154 1%
0/577 0%
Small Cell Lung Carcinoma
0/9 0%
2/752 0%
Squamous Cell Lung Carcinoma
0/57 0%
2/810 0%
Hepatocellular Carcinoma
0/46 0%
5/2210 0%
Wilms Tumour
0/5 0%
1/474 0%
Kidney Carcinoma
0/85 0%
4/1862 0%
Esophageal Carcinoma
0/23 0%
1/769 0%
Biliary Tract Carcinoma
0/54 0%
1/950 0%
Pancreatic Carcinoma
0/89 0%
1/1611 0%
Head and Neck Carcinoma
0/85 0%
1/1574 0%
Esophageal Squamous Cell Carcinoma
0/51 0%
1/2550 0%
B-Cell Non-Hodgkins Lymphoma
0/88 0%
1/2534 0%

Mutation Distribution

Where PGK1 is mutated · all tissues, split by cell line vs tissue

Mutation counts by tissue
Mutation Distribution

How many mutations in PGK1 were found in each tissue, across the whole database.

Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.

This shows the cancer-context where this gene is recurrently altered.

GTEx Expression

Median TPM across 54 healthy tissues

GTEx Portal ↗
About the expression data
GTEx Expression

Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.

Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.

Scroll or drag the mini-axis below the chart to browse all tissues.

Mutations

All 314 mutations in PGK1

About the mutation list
Mutations

Every mutation record for this gene, across all samples and sources.

The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).

Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.

IDSampleTranscriptAA Change CDSTypeSourceMutant PeptideWild-type Peptide