Stats by Source
Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)
Total = all mutations for this gene across every source.
Cell line = COSMIC Cell Lines Project + DepMap + PubMed.
Tissue = COSMIC primary-tissue (patient tumour) samples.
Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.
| Total | Cell line | Tissue | |
|---|---|---|---|
| Mutations | 855 | 126 | 722 |
| Samples | 244 | 50 | 190 |
| Peptides | 234 | 41 | 197 |
Function
PKM · Pyruvate kinase M1/2
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011].
Isoforms & Proteins
6 transcripts · UniProt mapping is sequence-verified (AA-safe)
Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.
The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.
Counts are mutations and unique mutant peptides on each transcript.
| Transcript | UniProt | Mutations | Peptides |
|---|---|---|---|
| ENST00000335181 | P14618 | 261 | 196 |
| ENST00000319622 | P14618-2 | 206 | 161 |
| ENST00000568459 | P14618-2 | 201 | 159 |
| ENST00000389093 | A0A0A0MRZ0* | 167 | 128 |
| ENST00000565154 | A0A804F729* | 16 | 11 |
| ENST00000565184 | A0A804F6T5* | 4 | 4 |
Gene Properties
Recurrent Mutations
All 196 amino-acid changes on canonical ENST00000335181 · needle height = samples · drag the mini-map to zoom
A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).
X-axis = amino-acid position in the protein.
Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.
The most recurrent changes are labelled; hover any needle for the change, position and counts.
Mutation frequency across cancer types
% of samples with a missense/complex mutation in PKM · cell line vs tissue
For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in PKM – counted as distinct samples (a sample counts once no matter how many mutations it has).
Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.
| Cancer type | Cell lines | Tissue samples |
|---|---|---|
| T-Lymphoblastic Leukemia | 4/40 10% | 0/0 0% |
| T-Cell Non-Hodgkins Lymphoma | 1/26 4% | 0/0 0% |
| Endometrial Carcinoma | 1/42 2% | 16/612 3% |
| Acute Myeloid Leukemia | 2/90 2% | 0/0 0% |
| Cervical Carcinoma | 1/35 3% | 6/422 1% |
| Squamous Cell Lung Carcinoma | 2/57 4% | 8/810 1% |
| Glioblastoma | 1/98 1% | 0/0 0% |
| Melanoma | 2/210 1% | 18/1899 1% |
| Colorectal Carcinoma | 7/143 5% | 24/3239 1% |
| Ovarian Carcinoma | 8/109 7% | 2/998 0% |
| Non-Small Cell Lung Carcinoma | 7/304 2% | 8/1390 1% |
| Burkitts Lymphoma | 1/32 3% | 1/196 1% |
| Gastric Carcinoma | 0/74 0% | 16/1809 1% |
| Hodgkins Lymphoma | 0/16 0% | 1/122 1% |
| Head and Neck Carcinoma | 0/85 0% | 12/1574 1% |
| Thyroid Gland Carcinoma | 1/45 2% | 9/1592 1% |
| Plasma Cell Myeloma | 2/44 5% | 0/305 0% |
| Non-Cancerous | 2/104 2% | 3/830 0% |
| Other Solid Cancers | 0/94 0% | 8/1515 1% |
| Bladder Carcinoma | 0/58 0% | 5/956 1% |
| Rhabdomyosarcoma | 1/33 3% | 0/171 0% |
| Mesothelioma | 1/62 2% | 0/165 0% |
| Small Cell Lung Carcinoma | 0/9 0% | 3/752 0% |
| Breast Carcinoma | 0/144 0% | 12/3264 0% |
| Hepatocellular Carcinoma | 0/46 0% | 7/2210 0% |
| Esophageal Squamous Cell Carcinoma | 1/51 2% | 7/2550 0% |
| Pancreatic Carcinoma | 1/89 1% | 4/1611 0% |
| Neuroblastoma | 2/87 2% | 2/1331 0% |
| Glioma | 0/52 0% | 6/2127 0% |
| Kidney Carcinoma | 0/85 0% | 5/1862 0% |
Mutation Distribution
Where PKM is mutated · all tissues, split by cell line vs tissue
How many mutations in PKM were found in each tissue, across the whole database.
Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.
This shows the cancer-context where this gene is recurrently altered.
GTEx Expression
Median TPM across 54 healthy tissues
Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.
Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.
Scroll or drag the mini-axis below the chart to browse all tissues.
Mutations
All 855 mutations in PKM
Every mutation record for this gene, across all samples and sources.
The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).
Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.
| ID | Sample | Transcript | AA Change | CDS | Type | Source | Mutant Peptide | Wild-type Peptide |
|---|