Stats by Source
Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)
Total = all mutations for this gene across every source.
Cell line = COSMIC Cell Lines Project + DepMap + PubMed.
Tissue = COSMIC primary-tissue (patient tumour) samples.
Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.
| Total | Cell line | Tissue | |
|---|---|---|---|
| Mutations | 532 | 120 | 408 |
| Samples | 155 | 50 | 104 |
| Peptides | 114 | 33 | 83 |
Function
POLR2E · RNA polymerase II, I and III subunit E
This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2015].
Isoforms & Proteins
4 transcripts · UniProt mapping is sequence-verified (AA-safe)
Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.
The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.
Counts are mutations and unique mutant peptides on each transcript.
| Transcript | UniProt | Mutations | Peptides |
|---|---|---|---|
| ENST00000615234 | P19388 | 164 | 96 |
| ENST00000586746 | P19388 | 130 | 91 |
| ENST00000612655 | P19388 | 130 | 91 |
| ENST00000619917 | A0A087WVZ9* | 108 | 80 |
Gene Properties
Recurrent Mutations
All 96 amino-acid changes on canonical ENST00000615234 · needle height = samples · drag the mini-map to zoom
A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).
X-axis = amino-acid position in the protein.
Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.
The most recurrent changes are labelled; hover any needle for the change, position and counts.
Mutation frequency across cancer types
% of samples with a missense/complex mutation in POLR2E · cell line vs tissue
For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in POLR2E – counted as distinct samples (a sample counts once no matter how many mutations it has).
Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.
| Cancer type | Cell lines | Tissue samples |
|---|---|---|
| T-Lymphoblastic Leukemia | 8/40 20% | 0/0 0% |
| Chronic Myelogenous Leukemia | 2/25 8% | 0/0 0% |
| Gastrointestinal Stromal Tumour | 0/0 0% | 6/133 5% |
| Oral Cavity Carcinoma | 2/54 4% | 0/0 0% |
| Endometrial Carcinoma | 3/42 7% | 4/612 1% |
| Cervical Carcinoma | 2/35 6% | 2/422 0% |
| Bladder Carcinoma | 0/58 0% | 8/956 1% |
| Hodgkins Lymphoma | 0/16 0% | 1/122 1% |
| Colorectal Carcinoma | 7/143 5% | 16/3239 0% |
| Squamous Cell Lung Carcinoma | 1/57 2% | 4/810 0% |
| Other Solid Cancers | 0/94 0% | 8/1515 1% |
| Non-Small Cell Lung Carcinoma | 3/304 1% | 5/1390 0% |
| Melanoma | 4/210 2% | 6/1899 0% |
| Mesothelioma | 1/62 2% | 0/165 0% |
| Esophageal Squamous Cell Carcinoma | 5/51 10% | 6/2550 0% |
| Neuroendocrine Tumour | 3/154 2% | 0/577 0% |
| Esophageal Carcinoma | 0/23 0% | 3/769 0% |
| Kidney Carcinoma | 3/85 4% | 4/1862 0% |
| Gastric Carcinoma | 1/74 1% | 5/1809 0% |
| Ewings Sarcoma | 0/63 0% | 1/262 0% |
| Breast Carcinoma | 4/144 3% | 5/3264 0% |
| Thyroid Gland Carcinoma | 0/45 0% | 3/1592 0% |
| Glioma | 0/52 0% | 4/2127 0% |
| Small Cell Lung Carcinoma | 0/9 0% | 1/752 0% |
| Hepatocellular Carcinoma | 0/46 0% | 3/2210 0% |
| Pancreatic Carcinoma | 1/89 1% | 1/1611 0% |
| Head and Neck Carcinoma | 0/85 0% | 2/1574 0% |
| Non-Cancerous | 0/104 0% | 1/830 0% |
| Ovarian Carcinoma | 0/109 0% | 1/998 0% |
| B-Cell Non-Hodgkins Lymphoma | 0/88 0% | 2/2534 0% |
Mutation Distribution
Where POLR2E is mutated · all tissues, split by cell line vs tissue
How many mutations in POLR2E were found in each tissue, across the whole database.
Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.
This shows the cancer-context where this gene is recurrently altered.
GTEx Expression
Median TPM across 54 healthy tissues
Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.
Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.
Scroll or drag the mini-axis below the chart to browse all tissues.
Mutations
All 532 mutations in POLR2E
Every mutation record for this gene, across all samples and sources.
The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).
Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.
| ID | Sample | Transcript | AA Change | CDS | Type | Source | Mutant Peptide | Wild-type Peptide |
|---|