Stats by Source
Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)
Total = all mutations for this gene across every source.
Cell line = COSMIC Cell Lines Project + DepMap + PubMed.
Tissue = COSMIC primary-tissue (patient tumour) samples.
Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.
| Total | Cell line | Tissue | |
|---|---|---|---|
| Mutations | 2,693 | 412 | 2,267 |
| Samples | 378 | 87 | 288 |
| Peptides | 393 | 77 | 332 |
Function
RASGRP4 · RAS guanyl releasing protein 4
The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009].
Isoforms & Proteins
8 transcripts · UniProt mapping is sequence-verified (AA-safe)
Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.
The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.
Counts are mutations and unique mutant peptides on each transcript.
Gene Properties
Recurrent Mutations
All 290 amino-acid changes on canonical ENST00000615439 · needle height = samples · drag the mini-map to zoom
A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).
X-axis = amino-acid position in the protein.
Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.
The most recurrent changes are labelled; hover any needle for the change, position and counts.
Mutation frequency across cancer types
% of samples with a missense/complex mutation in RASGRP4 · cell line vs tissue
For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in RASGRP4 – counted as distinct samples (a sample counts once no matter how many mutations it has).
Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.
| Cancer type | Cell lines | Tissue samples |
|---|---|---|
| Chronic Myelogenous Leukemia | 2/25 8% | 0/0 0% |
| T-Lymphoblastic Leukemia | 2/40 5% | 0/0 0% |
| Gastrointestinal Stromal Tumour | 0/0 0% | 6/133 5% |
| Oral Cavity Carcinoma | 2/54 4% | 0/0 0% |
| Chondrosarcoma | 2/14 14% | 1/75 1% |
| Endometrial Carcinoma | 4/42 10% | 15/612 2% |
| Melanoma | 7/210 3% | 41/1899 2% |
| Acute Myeloid Leukemia | 2/90 2% | 0/0 0% |
| Cervical Carcinoma | 0/35 0% | 7/422 2% |
| Osteosarcoma | 3/45 7% | 0/166 0% |
| Pheochromocytoma and Paraganglioma | 0/0 0% | 1/71 1% |
| Non-Small Cell Lung Carcinoma | 7/304 2% | 15/1390 1% |
| Squamous Cell Lung Carcinoma | 0/57 0% | 11/810 1% |
| Gastric Carcinoma | 3/74 4% | 19/1809 1% |
| Glioblastoma | 1/98 1% | 0/0 0% |
| Hepatocellular Carcinoma | 3/46 7% | 19/2210 1% |
| Colorectal Carcinoma | 8/143 6% | 24/3239 1% |
| Other Solid Cancers | 1/94 1% | 14/1515 1% |
| Esophageal Squamous Cell Carcinoma | 0/51 0% | 23/2550 1% |
| Mesothelioma | 2/62 3% | 0/165 0% |
| Plasma Cell Myeloma | 1/44 2% | 2/305 1% |
| Hodgkins Lymphoma | 0/16 0% | 1/122 1% |
| Biliary Tract Carcinoma | 2/54 4% | 5/950 1% |
| Bladder Carcinoma | 2/58 3% | 5/956 1% |
| Thyroid Gland Carcinoma | 3/45 7% | 7/1592 0% |
| Small Cell Lung Carcinoma | 0/9 0% | 4/752 1% |
| Other Sarcomas | 3/69 4% | 1/699 0% |
| Germ Cell Tumour | 0/25 0% | 1/169 1% |
| Kidney Carcinoma | 3/85 4% | 7/1862 0% |
| Esophageal Carcinoma | 0/23 0% | 4/769 1% |
Mutation Distribution
Where RASGRP4 is mutated · all tissues, split by cell line vs tissue
How many mutations in RASGRP4 were found in each tissue, across the whole database.
Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.
This shows the cancer-context where this gene is recurrently altered.
GTEx Expression
Median TPM across 54 healthy tissues
Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.
Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.
Scroll or drag the mini-axis below the chart to browse all tissues.
Mutations
All 2,693 mutations in RASGRP4
Every mutation record for this gene, across all samples and sources.
The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).
Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.
| ID | Sample | Transcript | AA Change | CDS | Type | Source | Mutant Peptide | Wild-type Peptide |
|---|