Stats by Source
Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)
Total = all mutations for this gene across every source.
Cell line = COSMIC Cell Lines Project + DepMap + PubMed.
Tissue = COSMIC primary-tissue (patient tumour) samples.
Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.
| Total | Cell line | Tissue | |
|---|---|---|---|
| Mutations | 1,358 | 199 | 1,151 |
| Samples | 531 | 121 | 403 |
| Peptides | 449 | 74 | 381 |
Function
REST · RE1 silencing transcription factor
This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018].
Isoforms & Proteins
8 transcripts · UniProt mapping is sequence-verified (AA-safe)
Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.
The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.
Counts are mutations and unique mutant peptides on each transcript.
| Transcript | UniProt | Mutations | Peptides |
|---|---|---|---|
| ENST00000309042 | Q13127 | 573 | 386 |
| ENST00000640343 | L0B1V4* | 119 | 103 |
| ENST00000616975 | A0A087X1C2* | 116 | 100 |
| ENST00000611211 | A0A087WV36* | 115 | 99 |
| ENST00000619101 | A0A087X1C2* | 115 | 99 |
| ENST00000638187 | A0A1W2PQA1* | 107 | 91 |
| ENST00000640168 | L0B3M6* | 107 | 91 |
| ENST00000622863 | A0A087WYA5* | 106 | 94 |
Gene Properties
Recurrent Mutations
All 385 amino-acid changes on canonical ENST00000309042 · needle height = samples · drag the mini-map to zoom
A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).
X-axis = amino-acid position in the protein.
Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.
The most recurrent changes are labelled; hover any needle for the change, position and counts.
Mutation frequency across cancer types
% of samples with a missense/complex mutation in REST · cell line vs tissue
For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in REST – counted as distinct samples (a sample counts once no matter how many mutations it has).
Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.
| Cancer type | Cell lines | Tissue samples |
|---|---|---|
| Chronic Myelogenous Leukemia | 2/25 8% | 0/0 0% |
| Endometrial Carcinoma | 9/42 21% | 26/612 4% |
| Gastrointestinal Stromal Tumour | 0/0 0% | 6/133 5% |
| Melanoma | 8/210 4% | 53/1899 3% |
| T-Lymphoblastic Leukemia | 1/40 2% | 0/0 0% |
| Non-Small Cell Lung Carcinoma | 22/304 7% | 19/1390 1% |
| Squamous Cell Lung Carcinoma | 0/57 0% | 19/810 2% |
| Hodgkins Lymphoma | 2/16 12% | 1/122 1% |
| Glioblastoma | 2/98 2% | 0/0 0% |
| Cervical Carcinoma | 2/35 6% | 7/422 2% |
| Colorectal Carcinoma | 15/143 10% | 48/3239 1% |
| Gastric Carcinoma | 1/74 1% | 33/1809 2% |
| Bladder Carcinoma | 4/58 7% | 14/956 1% |
| Adrenocortical Carcinoma | 0/3 0% | 2/112 2% |
| Germ Cell Tumour | 2/25 8% | 1/169 1% |
| Osteosarcoma | 2/45 4% | 1/166 1% |
| Other Solid Cancers | 2/94 2% | 18/1515 1% |
| Ovarian Carcinoma | 4/109 4% | 8/998 1% |
| Hepatocellular Carcinoma | 1/46 2% | 22/2210 1% |
| Small Cell Lung Carcinoma | 1/9 11% | 6/752 1% |
| Ewings Sarcoma | 0/63 0% | 3/262 1% |
| Prostate Carcinoma | 0/13 0% | 19/2105 1% |
| Head and Neck Carcinoma | 2/85 2% | 13/1574 1% |
| Plasma Cell Myeloma | 2/44 5% | 1/305 0% |
| Neuroendocrine Tumour | 3/154 2% | 3/577 1% |
| Other Sarcomas | 2/69 3% | 4/699 1% |
| Neuroblastoma | 4/87 5% | 6/1331 0% |
| Medulloblastoma | 0/0 0% | 3/450 1% |
| Non-Cancerous | 0/104 0% | 6/830 1% |
| Biliary Tract Carcinoma | 2/54 4% | 4/950 0% |
Mutation Distribution
Where REST is mutated · all tissues, split by cell line vs tissue
How many mutations in REST were found in each tissue, across the whole database.
Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.
This shows the cancer-context where this gene is recurrently altered.
GTEx Expression
Median TPM across 54 healthy tissues
Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.
Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.
Scroll or drag the mini-axis below the chart to browse all tissues.
Mutations
All 1,358 mutations in REST
Every mutation record for this gene, across all samples and sources.
The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).
Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.
| ID | Sample | Transcript | AA Change | CDS | Type | Source | Mutant Peptide | Wild-type Peptide |
|---|