RFXAP

Regulatory factor X associated protein O00287 RFXAP_HUMAN
Protein Coding Chr 13 13q13.3 Swiss-Prot reviewed Entrez 5994
Mutations
85
CL 20 · Tissue 65
Samples
83
CL 19 · Tissue 64
Peptides
64
unique mutant peptides
Transcripts
1
isoforms mutated

Stats by Source

Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)

How the counts split by source
Stats by Source

Total = all mutations for this gene across every source.

Cell line = COSMIC Cell Lines Project + DepMap + PubMed.

Tissue = COSMIC primary-tissue (patient tumour) samples.

Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.

TotalCell lineTissue
Mutations852065
Samples831964
Peptides641351

Function

RFXAP · Regulatory factor X associated protein

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated ankyrin-containing protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group D. Transcript variants utilizing different polyA signals have been found for this gene. [provided by RefSeq, Jul 2008].

Isoforms & Proteins

1 transcript · UniProt mapping is sequence-verified (AA-safe)

About the isoform mapping
Isoforms & Proteins

Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.

The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.

Counts are mutations and unique mutant peptides on each transcript.

TranscriptUniProtMutationsPeptides
ENST00000255476 O00287 85 64

Gene Properties

Type
Protein Coding
Chromosome
13
Cytoband
13q13.3
Entrez ID
Aliases
MHC2D4

Recurrent Mutations

All 64 amino-acid changes on canonical ENST00000255476 · needle height = samples · drag the mini-map to zoom

What this lollipop shows
Recurrent Mutations

A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).

X-axis = amino-acid position in the protein.

Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.

The most recurrent changes are labelled; hover any needle for the change, position and counts.

Mutation frequency across cancer types

% of samples with a missense/complex mutation in RFXAP · cell line vs tissue

How this frequency is counted
Cancer-type mutation frequency

For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in RFXAP – counted as distinct samples (a sample counts once no matter how many mutations it has).

Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.

Cancer typeCell linesTissue samples
T-Lymphoblastic Leukemia
1/40 2%
0/0 0%
Endometrial Carcinoma
2/42 5%
5/612 1%
Hodgkins Lymphoma
0/16 0%
1/122 1%
Medulloblastoma
0/0 0%
2/450 0%
Cervical Carcinoma
0/35 0%
2/422 0%
Non-Small Cell Lung Carcinoma
5/304 2%
2/1390 0%
Bladder Carcinoma
1/58 2%
3/956 0%
Colorectal Carcinoma
4/143 3%
8/3239 0%
Thyroid Gland Carcinoma
0/45 0%
5/1592 0%
Ewings Sarcoma
1/63 2%
0/262 0%
Plasma Cell Myeloma
1/44 2%
0/305 0%
Other Sarcomas
0/69 0%
2/699 0%
Small Cell Lung Carcinoma
0/9 0%
2/752 0%
Esophageal Carcinoma
0/23 0%
2/769 0%
Squamous Cell Lung Carcinoma
1/57 2%
1/810 0%
B-Cell Non-Hodgkins Lymphoma
3/88 3%
2/2534 0%
Hepatocellular Carcinoma
0/46 0%
4/2210 0%
Head and Neck Carcinoma
0/85 0%
3/1574 0%
Gastric Carcinoma
0/74 0%
3/1809 0%
Melanoma
0/210 0%
3/1899 0%
Neuroendocrine Tumour
0/154 0%
1/577 0%
Esophageal Squamous Cell Carcinoma
0/51 0%
3/2550 0%
Breast Carcinoma
0/144 0%
4/3264 0%
Other Solid Cancers
0/94 0%
2/1515 0%
Kidney Carcinoma
0/85 0%
2/1862 0%
Biliary Tract Carcinoma
0/54 0%
1/950 0%
Glioma
0/52 0%
1/2127 0%

Mutation Distribution

Where RFXAP is mutated · all tissues, split by cell line vs tissue

Mutation counts by tissue
Mutation Distribution

How many mutations in RFXAP were found in each tissue, across the whole database.

Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.

This shows the cancer-context where this gene is recurrently altered.

GTEx Expression

Median TPM across 54 healthy tissues

GTEx Portal ↗
About the expression data
GTEx Expression

Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.

Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.

Scroll or drag the mini-axis below the chart to browse all tissues.

Mutations

All 85 mutations in RFXAP

About the mutation list
Mutations

Every mutation record for this gene, across all samples and sources.

The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).

Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.

IDSampleTranscriptAA Change CDSTypeSourceMutant PeptideWild-type Peptide