Stats by Source
Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)
Total = all mutations for this gene across every source.
Cell line = COSMIC Cell Lines Project + DepMap + PubMed.
Tissue = COSMIC primary-tissue (patient tumour) samples.
Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.
| Total | Cell line | Tissue | |
|---|---|---|---|
| Mutations | 648 | 136 | 501 |
| Samples | 560 | 117 | 436 |
| Peptides | 453 | 80 | 379 |
Function
ROCK1 · Rho associated coiled-coil containing protein kinase 1
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015].
Isoforms & Proteins
2 transcripts · UniProt mapping is sequence-verified (AA-safe)
Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.
The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.
Counts are mutations and unique mutant peptides on each transcript.
| Transcript | UniProt | Mutations | Peptides |
|---|---|---|---|
| ENST00000399799 | Q13464 | 646 | 451 |
| ENST00000635540 | A0A0U1RQV4* | 2 | 2 |
Gene Properties
Recurrent Mutations
All 451 amino-acid changes on canonical ENST00000399799 · needle height = samples · drag the mini-map to zoom
A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).
X-axis = amino-acid position in the protein.
Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.
The most recurrent changes are labelled; hover any needle for the change, position and counts.
Mutation frequency across cancer types
% of samples with a missense/complex mutation in ROCK1 · cell line vs tissue
For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in ROCK1 – counted as distinct samples (a sample counts once no matter how many mutations it has).
Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.
| Cancer type | Cell lines | Tissue samples |
|---|---|---|
| Chronic Myelogenous Leukemia | 3/25 12% | 0/0 0% |
| T-Lymphoblastic Leukemia | 4/40 10% | 0/0 0% |
| Endometrial Carcinoma | 7/42 17% | 29/612 5% |
| Acute Myeloid Leukemia | 4/90 4% | 0/0 0% |
| Oral Cavity Carcinoma | 2/54 4% | 0/0 0% |
| Meningioma | 2/3 67% | 7/252 3% |
| Osteosarcoma | 5/45 11% | 1/166 1% |
| Unknown | 0/10 0% | 1/29 3% |
| Non-Small Cell Lung Carcinoma | 14/304 5% | 29/1390 2% |
| Colorectal Carcinoma | 19/143 13% | 62/3239 2% |
| Hodgkins Lymphoma | 2/16 12% | 1/122 1% |
| Glioblastoma | 2/98 2% | 0/0 0% |
| Gastric Carcinoma | 0/74 0% | 33/1809 2% |
| Melanoma | 3/210 1% | 34/1899 2% |
| Squamous Cell Lung Carcinoma | 3/57 5% | 12/810 1% |
| Bladder Carcinoma | 0/58 0% | 17/956 2% |
| Cervical Carcinoma | 2/35 6% | 5/422 1% |
| Head and Neck Carcinoma | 5/85 6% | 19/1574 1% |
| Burkitts Lymphoma | 0/32 0% | 3/196 2% |
| Other Solid Cancers | 1/94 1% | 19/1515 1% |
| Ewings Sarcoma | 4/63 6% | 0/262 0% |
| Biliary Tract Carcinoma | 1/54 2% | 11/950 1% |
| Thyroid Gland Carcinoma | 2/45 4% | 17/1592 1% |
| Chondrosarcoma | 1/14 7% | 0/75 0% |
| Neuroendocrine Tumour | 5/154 3% | 3/577 1% |
| Hepatocellular Carcinoma | 0/46 0% | 24/2210 1% |
| Small Cell Lung Carcinoma | 0/9 0% | 8/752 1% |
| Rhabdomyosarcoma | 1/33 3% | 1/171 1% |
| Prostate Carcinoma | 5/13 38% | 15/2105 1% |
| Plasma Cell Myeloma | 1/44 2% | 2/305 1% |
Mutation Distribution
Where ROCK1 is mutated · all tissues, split by cell line vs tissue
How many mutations in ROCK1 were found in each tissue, across the whole database.
Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.
This shows the cancer-context where this gene is recurrently altered.
GTEx Expression
Median TPM across 54 healthy tissues
Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.
Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.
Scroll or drag the mini-axis below the chart to browse all tissues.
Mutations
All 648 mutations in ROCK1
Every mutation record for this gene, across all samples and sources.
The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).
Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.
| ID | Sample | Transcript | AA Change | CDS | Type | Source | Mutant Peptide | Wild-type Peptide |
|---|