Stats by Source
Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)
Total = all mutations for this gene across every source.
Cell line = COSMIC Cell Lines Project + DepMap + PubMed.
Tissue = COSMIC primary-tissue (patient tumour) samples.
Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.
| Total | Cell line | Tissue | |
|---|---|---|---|
| Mutations | 2,408 | 253 | 2,130 |
| Samples | 481 | 79 | 397 |
| Peptides | 331 | 50 | 293 |
Function
SPAM1 · Sperm adhesion molecule 1
Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010].
Isoforms & Proteins
6 transcripts · UniProt mapping is sequence-verified (AA-safe)
Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.
The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.
Counts are mutations and unique mutant peptides on each transcript.
Gene Properties
Recurrent Mutations
All 311 amino-acid changes on canonical ENST00000223028 · needle height = samples · drag the mini-map to zoom
A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).
X-axis = amino-acid position in the protein.
Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.
The most recurrent changes are labelled; hover any needle for the change, position and counts.
Mutation frequency across cancer types
% of samples with a missense/complex mutation in SPAM1 · cell line vs tissue
For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in SPAM1 – counted as distinct samples (a sample counts once no matter how many mutations it has).
Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.
| Cancer type | Cell lines | Tissue samples |
|---|---|---|
| T-Cell Non-Hodgkins Lymphoma | 4/26 15% | 0/0 0% |
| Chronic Myelogenous Leukemia | 3/25 12% | 0/0 0% |
| Melanoma | 10/210 5% | 96/1899 5% |
| Endometrial Carcinoma | 3/42 7% | 21/612 3% |
| Squamous Cell Lung Carcinoma | 7/57 12% | 18/810 2% |
| Other Solid Cancers | 1/94 1% | 45/1515 3% |
| Non-Small Cell Lung Carcinoma | 8/304 3% | 34/1390 2% |
| Small Cell Lung Carcinoma | 0/9 0% | 12/752 2% |
| Neuroendocrine Tumour | 6/154 4% | 4/577 1% |
| Ovarian Carcinoma | 6/109 6% | 9/998 1% |
| Colorectal Carcinoma | 2/143 1% | 35/3239 1% |
| Glioblastoma | 1/98 1% | 0/0 0% |
| Head and Neck Carcinoma | 3/85 4% | 14/1574 1% |
| Rhabdomyosarcoma | 2/33 6% | 0/171 0% |
| Gastric Carcinoma | 3/74 4% | 15/1809 1% |
| Esophageal Squamous Cell Carcinoma | 3/51 6% | 21/2550 1% |
| Adrenocortical Carcinoma | 0/3 0% | 1/112 1% |
| Glioma | 2/52 4% | 13/2127 1% |
| Other Sarcomas | 0/69 0% | 5/699 1% |
| Kidney Carcinoma | 1/85 1% | 11/1862 1% |
| Hepatocellular Carcinoma | 2/46 4% | 10/2210 0% |
| Osteosarcoma | 0/45 0% | 1/166 1% |
| Mesothelioma | 1/62 2% | 0/165 0% |
| Esophageal Carcinoma | 0/23 0% | 3/769 0% |
| B-Cell Non-Hodgkins Lymphoma | 5/88 6% | 4/2534 0% |
| Breast Carcinoma | 2/144 1% | 9/3264 0% |
| Bladder Carcinoma | 1/58 2% | 2/956 0% |
| Plasma Cell Myeloma | 1/44 2% | 0/305 0% |
| Cervical Carcinoma | 0/35 0% | 1/422 0% |
| Medulloblastoma | 0/0 0% | 1/450 0% |
Mutation Distribution
Where SPAM1 is mutated · all tissues, split by cell line vs tissue
How many mutations in SPAM1 were found in each tissue, across the whole database.
Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.
This shows the cancer-context where this gene is recurrently altered.
GTEx Expression
Median TPM across 3 healthy tissues
Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.
Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.
Scroll or drag the mini-axis below the chart to browse all tissues.
Mutations
All 2,408 mutations in SPAM1
Every mutation record for this gene, across all samples and sources.
The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).
Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.
| ID | Sample | Transcript | AA Change | CDS | Type | Source | Mutant Peptide | Wild-type Peptide |
|---|