Stats by Source
Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)
Total = all mutations for this gene across every source.
Cell line = COSMIC Cell Lines Project + DepMap + PubMed.
Tissue = COSMIC primary-tissue (patient tumour) samples.
Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.
| Total | Cell line | Tissue | |
|---|---|---|---|
| Mutations | 11,911 | 982 | 10,858 |
| Samples | 521 | 79 | 437 |
| Peptides | 567 | 68 | 511 |
Function
TCF4 · Transcription factor 4
This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Jul 2016].
Isoforms & Proteins
35 transcripts · UniProt mapping is sequence-verified (AA-safe)
Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.
The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.
Counts are mutations and unique mutant peptides on each transcript.
| Transcript | UniProt | Mutations | Peptides |
|---|---|---|---|
| ENST00000398339 | E9PH57* | 489 | 352 |
| ENST00000354452 | P15884-3 | 477 | 341 |
| ENST00000564403 | H3BTP3* | 435 | 328 |
| ENST00000629387 | P15884-3 | 431 | 324 |
| ENST00000356073 | P15884 | 428 | 321 |
| ENST00000564999 | P15884 | 428 | 321 |
| ENST00000566286 | P15884-12 | 423 | 318 |
| ENST00000537578 | P15884-13 | 420 | 315 |
| ENST00000568673 | P15884-13 | 420 | 315 |
| ENST00000636400 | P15884-13 | 420 | 315 |
| ENST00000540999 | P15884-14 | 417 | 312 |
| ENST00000568740 | H3BPJ7* | 417 | 312 |
| ENST00000543082 | P15884-10 | 412 | 307 |
| ENST00000544241 | P15884-11 | 396 | 290 |
| ENST00000564228 | P15884-7 | 393 | 287 |
| ENST00000566279 | P15884-4 | 391 | 295 |
| ENST00000567880 | P15884-5 | 388 | 292 |
| ENST00000635822 | A0A1B0GVB8* | 384 | 291 |
| ENST00000565018 | P15884-15 | 377 | 283 |
| ENST00000616053 | P15884-15 | 377 | 283 |
| ENST00000636822 | A0A1B0GWD5* | 372 | 273 |
| ENST00000643689 | A0A1B0GWD5* | 372 | 273 |
| ENST00000537856 | P15884-9 | 369 | 270 |
| ENST00000561992 | P15884-9 | 369 | 270 |
| ENST00000570177 | P15884-9 | 369 | 270 |
| ENST00000457482 | P15884-2 | 341 | 252 |
| ENST00000561831 | P15884-8 | 339 | 250 |
| ENST00000570287 | P15884-6 | 338 | 249 |
| ENST00000637169 | A0A1B0GW91* | 311 | 233 |
| ENST00000626584 | P15884-16 | 308 | 230 |
| ENST00000638154 | A0A1B0GVR6* | 42 | 32 |
| ENST00000355995 | Q9NQB0 | 23 | 16 |
| ENST00000355717 | Q9NQB0-11 | 16 | 12 |
| ENST00000369397 | Q9NQB0-8 | 14 | 11 |
| ENST00000627217 | Q9NQB0-7 | 5 | 5 |
Gene Properties
Recurrent Mutations
All 341 amino-acid changes on canonical ENST00000354452 · needle height = samples · drag the mini-map to zoom
A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).
X-axis = amino-acid position in the protein.
Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.
The most recurrent changes are labelled; hover any needle for the change, position and counts.
Mutation frequency across cancer types
% of samples with a missense/complex mutation in TCF4 · cell line vs tissue
For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in TCF4 – counted as distinct samples (a sample counts once no matter how many mutations it has).
Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.
| Cancer type | Cell lines | Tissue samples |
|---|---|---|
| T-Lymphoblastic Leukemia | 6/40 15% | 0/0 0% |
| Melanoma | 17/210 8% | 106/1899 6% |
| Endometrial Carcinoma | 2/42 5% | 22/612 4% |
| Gastrointestinal Stromal Tumour | 0/0 0% | 3/133 2% |
| Hodgkins Lymphoma | 2/16 12% | 1/122 1% |
| Colorectal Carcinoma | 13/143 9% | 60/3239 2% |
| Glioblastoma | 2/98 2% | 0/0 0% |
| Non-Small Cell Lung Carcinoma | 6/304 2% | 28/1390 2% |
| Germ Cell Tumour | 0/25 0% | 3/169 2% |
| Squamous Cell Lung Carcinoma | 1/57 2% | 12/810 1% |
| Gastric Carcinoma | 2/74 3% | 24/1809 1% |
| Other Sarcomas | 3/69 4% | 6/699 1% |
| Other Solid Cancers | 2/94 2% | 16/1515 1% |
| Neuroendocrine Tumour | 3/154 2% | 4/577 1% |
| Hepatocellular Carcinoma | 3/46 7% | 17/2210 1% |
| Biliary Tract Carcinoma | 1/54 2% | 7/950 1% |
| Bladder Carcinoma | 0/58 0% | 8/956 1% |
| Esophageal Carcinoma | 0/23 0% | 6/769 1% |
| Cervical Carcinoma | 0/35 0% | 3/422 1% |
| Esophageal Squamous Cell Carcinoma | 2/51 4% | 15/2550 1% |
| Non-Cancerous | 0/104 0% | 6/830 1% |
| B-Cell Non-Hodgkins Lymphoma | 1/88 1% | 15/2534 1% |
| Thyroid Gland Carcinoma | 0/45 0% | 10/1592 1% |
| Plasma Cell Myeloma | 0/44 0% | 2/305 1% |
| Breast Carcinoma | 2/144 1% | 17/3264 1% |
| Glioma | 0/52 0% | 12/2127 1% |
| Head and Neck Carcinoma | 2/85 2% | 7/1574 0% |
| Small Cell Lung Carcinoma | 0/9 0% | 4/752 1% |
| Prostate Carcinoma | 3/13 23% | 8/2105 0% |
| Rhabdomyosarcoma | 0/33 0% | 1/171 1% |
Mutation Distribution
Where TCF4 is mutated · all tissues, split by cell line vs tissue
How many mutations in TCF4 were found in each tissue, across the whole database.
Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.
This shows the cancer-context where this gene is recurrently altered.
GTEx Expression
Median TPM across 54 healthy tissues
Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.
Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.
Scroll or drag the mini-axis below the chart to browse all tissues.
Mutations
All 11,911 mutations in TCF4
Every mutation record for this gene, across all samples and sources.
The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).
Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.
| ID | Sample | Transcript | AA Change | CDS | Type | Source | Mutant Peptide | Wild-type Peptide |
|---|