U2AF1

U2 small nuclear RNA auxiliary factor 1 Q01081 U2AF1_HUMAN
Protein Coding Chr 21 21q22.3 Swiss-Prot reviewed Entrez 7307
Mutations
768
CL 39 · Tissue 723
Samples
318
CL 19 · Tissue 297
Peptides
132
unique mutant peptides
Transcripts
3
isoforms mutated

Stats by Source

Total, split by cell line (COSMIC CL / DepMap / PubMed) vs tissue (COSMIC primary tissue)

How the counts split by source
Stats by Source

Total = all mutations for this gene across every source.

Cell line = COSMIC Cell Lines Project + DepMap + PubMed.

Tissue = COSMIC primary-tissue (patient tumour) samples.

Total can exceed cell line + tissue: COSMIC tissue-derived models sit only in global, and a peptide can be shared across both.

TotalCell lineTissue
Mutations76839723
Samples31819297
Peptides13210123

Function

U2AF1 · U2 small nuclear RNA auxiliary factor 1

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].

Isoforms & Proteins

3 transcripts · UniProt mapping is sequence-verified (AA-safe)

About the isoform mapping
Isoforms & Proteins

Each Ensembl transcript (ENST) this gene is mutated on, with its matched UniProt accession.

The mapping is sequence-verified: the UniProt sequence is identical to the transcript translation, so amino-acid positions line up exactly. A * marks an unreviewed (TrEMBL) entry.

Counts are mutations and unique mutant peptides on each transcript.

TranscriptUniProtMutationsPeptides
ENST00000291552 Q01081 313 112
ENST00000380276 Q01081-2 309 108
ENST00000459639 Q01081-4 146 78

Gene Properties

Type
Protein Coding
Chromosome
21
Cytoband
21q22.3
Entrez ID
Aliases
FP793RNRNU2AF1U2AF35U2AFBP

Recurrent Mutations

All 108 amino-acid changes on canonical ENST00000380276 · needle height = samples · drag the mini-map to zoom

What this lollipop shows
Recurrent Mutations

A lollipop / needle plot – the standard way to show recurrent mutations along a protein (as used by cBioPortal and MutationMapper).

X-axis = amino-acid position in the protein.

Needle height & head size = how often that exact amino-acid change was observed (its recurrence). Tall/large heads are mutational hotspots.

The most recurrent changes are labelled; hover any needle for the change, position and counts.

Mutation frequency across cancer types

% of samples with a missense/complex mutation in U2AF1 · cell line vs tissue

How this frequency is counted
Cancer-type mutation frequency

For each cancer type, the fraction of samples that carry at least one missense/complex mutation anywhere in U2AF1 – counted as distinct samples (a sample counts once no matter how many mutations it has).

Split into cell line and tissue; each cell shows mutated / total and the percentage. Cohorts with <20 samples are omitted. Ordered by combined frequency.

Cancer typeCell linesTissue samples
Acute Myeloid Leukemia
4/90 4%
0/0 0%
Acute Monocytic Leukemia
0/1 0%
1/25 4%
Non-Small Cell Lung Carcinoma
9/304 3%
31/1390 2%
Other Blood Cancers
0/61 0%
62/2725 2%
Endometrial Carcinoma
1/42 2%
11/612 2%
Bladder Carcinoma
0/58 0%
16/956 2%
Colorectal Carcinoma
3/143 2%
35/3239 1%
Pancreatic Carcinoma
0/89 0%
17/1611 1%
Gastrointestinal Stromal Tumour
0/0 0%
1/133 1%
B-Lymphoblastic Leukemia
0/55 0%
20/2640 1%
Neuroendocrine Tumour
0/154 0%
5/577 1%
Small Cell Lung Carcinoma
0/9 0%
5/752 1%
Cervical Carcinoma
0/35 0%
3/422 1%
Melanoma
2/210 1%
11/1899 1%
Biliary Tract Carcinoma
0/54 0%
5/950 1%
Gastric Carcinoma
0/74 0%
9/1809 0%
Hepatocellular Carcinoma
0/46 0%
10/2210 0%
Glioma
0/52 0%
9/2127 0%
Other Sarcomas
0/69 0%
3/699 0%
Other Solid Cancers
0/94 0%
6/1515 0%
Head and Neck Carcinoma
0/85 0%
6/1574 0%
Prostate Carcinoma
0/13 0%
7/2105 0%
Ovarian Carcinoma
0/109 0%
3/998 0%
Esophageal Carcinoma
0/23 0%
2/769 0%
Squamous Cell Lung Carcinoma
0/57 0%
2/810 0%
Medulloblastoma
0/0 0%
1/450 0%
Non-Cancerous
0/104 0%
2/830 0%
Wilms Tumour
0/5 0%
1/474 0%
Thyroid Gland Carcinoma
0/45 0%
3/1592 0%
Breast Carcinoma
0/144 0%
6/3264 0%

Mutation Distribution

Where U2AF1 is mutated · all tissues, split by cell line vs tissue

Mutation counts by tissue
Mutation Distribution

How many mutations in U2AF1 were found in each tissue, across the whole database.

Each bar is a tissue (cell-line and tissue names are merged to the standard tissue), split into cell line and tissue (patient tumour) contributions.

This shows the cancer-context where this gene is recurrently altered.

GTEx Expression

Median TPM across 54 healthy tissues

GTEx Portal ↗
About the expression data
GTEx Expression

Median gene expression (TPM) in normal, non-cancer human tissues from the GTEx project.

Useful for judging tumour specificity – a strong neoantigen target ideally comes from a gene with low expression in healthy tissues.

Scroll or drag the mini-axis below the chart to browse all tissues.

Mutations

All 768 mutations in U2AF1

About the mutation list
Mutations

Every mutation record for this gene, across all samples and sources.

The Sample column links to the cell line (cell-line samples) or the tissue type (tissue samples).

Use the Type / Source filters, the search box, and column sorting to explore; each CAN-IMMUNE ID opens the full mutation & peptide view.

IDSampleTranscriptAA Change CDSTypeSourceMutant PeptideWild-type Peptide